Once the new imaging system was set up I started to develop my knowledge of digital imaging for science. I was surrounded by Phd's and peers with the need to incorporate imaging and image analysis techniques into their research.
The beginning of the digital dark room support at DuPont Merck Pharmaceuticals was an off shoot of my microscopy and histology skills. In order for me to convey what I saw through the scope I had relied on 35mm camera backs and the training I had at ATCC microscopy course taught by Mortimor Abramowitz.
The cost, time, and effort of film based images was the price my company had to pay until I went digital. The payback was immediate and rewarding. The epifluorescence work I was in the middle of conducting went from a turn around time of days and then sometime having to redo an entire experiment to being able to precisely visualize the optimum exposure and focus.
With turnaround time becoming instantaneous and the new ability to work with images directly on a monitor possibilities of using this resource to advance our pharmaceutical research grew.
Photoshop CS3 Extended
The new analysis menu item is only accessible if your have the Extended version of Photoshop CS3. Under this menu one can find calibration, data point selection, basic analysis tools, and the ability to automatically place a calibrated scale bar annotation in an image. Associated with the eyedropper tool are two analysis tools, the ruler tool and a new count tool.
Many researchers use Photoshop to optimize images for communication. Some, including myself, will incorporate Photoshop in their workflow to process images prior to analysis in dedicated image analysis software. My favorite is Image Pro Plus from Media Cybernetics. Now Photoshop analysis abilities will not supplant a dedicated program it can, for simpler analysis, process the entire workflow.
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